عملکرد آنزیم‌‌های گوارشی و متابولیسم‌های حد واسط شته سبز هلو، Sulzer) Myzus persicae) تحت تاثیر ریزمغذی‌های مختلف در شرایط گلخانه‌ای

Bell pepper (Capsicum annum L., Solanaceae) is an essential vegetable with a good source of vitamins, micronutrients and anti- oxidant that plays an important role in human health. This plant is attacked by several insect pests, including green peach aphid, Myzus persicae (Sulzer). In addition to direct damage, this pest significantly able to the transmit plant viruses such as potato leaf roll virus (PLRV), potato virus Y (PVY) and cucumber mosaic virus (CMV). In current research, the effects of micronutrients of iron, zinc sulfate, copper sulfate and manganese sulfate were investigated on digestive enzymes performance and intermediary metabolisms of M. persicae under greenhouse conditions. To investigate the enzymatic studies, a sufficient number of the aphid adults were selected randomly from per replication and treatment. Then, the samples were centrifuged at 13000×g for 15 min at 4 ºC and the supernatants was used as an enzymatic source. The results showed that the highest and lowest activity of trypsin and elastase were observed on M. persicae reared on ZnSo4 (15.87 and 14.63 U/mg protein) and MnSo4 (3.94 and 4.81 U/mg protein) treated plants, respectively. While, the highest and lowest activity of chymotrypsin was recorded on M. persicae reared on control and MnSo4 (12.34 and 4.99 U/mg protein) treated plants, respectively. The highest values of enzymes activity of amino- and carboxy- peptidase were obtained on ZnSo4 (17.19 and 25.11 U/mg protein, respectively) treatment and their lowest values were recorded on control and Fe (11.40 and 7.22 U/mg protein, respect.) treatments, respectively. Also, the highest and lowest activity of α- amylase had on Fe and MnSo4 (66.02 and 14.01 U/mg protein, respect.) treatments. The highest activities of aspartate – (AST) and alanine- aminotransferase (ALT) were obtained on M. persicae reared on ZnSo4 (1.113 and 2.113 U/mg protein) treated plants, respectively and the lowest these values were recorded on MnSo4 (0.027 and 0.527 U/mg protein) treatments. The highest and lowest activity of gamma glutamyl transferase (γ-GT) were observed on ZnSo4 (1.588 U/mg protein) and control (0.924 U/mg protein) treatment, respectively. The results showed that digestive enzymes performance and intermediary metabolisms of M. persicae significantly affected by changes in the quality of plant nutrient, which causes changes in the secondary metabolites contents mainly phenolic compounds. Based on this results, MnSo4 compared to other micronutrients due to its anti- feeding effects can reduce feeding activity of M. persicae and can be used in integrated pest management programs (IPM) of green peach aphid in greenhouses.