بررسی بازدارندگی عصاره‌های چندگیاه داروئی بر رشد قارچ Monilinia fructigenaعامل پوسیدگی قهوه‌ای درختان میوه هسته‌دار در شرایط آزمایشگاهی

Brown rot of stone fruit trees disease created by Monilinia spp. is always one of the most important and dangerous plant diseases of these trees in the world. Since the extracts of certain compounds of pharmaceutical herbs have antifungal properties. this study was carried out with the aim of investigating the inhibition effect of garlic, thyme, eucalyptus and oleander extract, on Monilinia fructigena's growth rate in vitro. It is noteworthy that the origin of this fungus is Golestan province and it has been identified with valid identification keys. Initially, the ethanolic extract of these plants were prepared and mixed with culture media. Then the inhibitory effect of them, were evaluated in different extract concentrations as 5, 10, 15 and 20 mg/l. This experiment was performed as a factorial in a completely randomized design with 2 factors and 3 replications. The first factor was the type of plant and the second factor was the level of extract concentration. The results of this research showed that between various plant extract and various doses and also interaction between them, there were significant differences on inhibition of growth rate of Monilia fructigena (p≥0/001). Also the results of this research showed that garlic and oleander extract with mean of %52/ 354 had the most inhibitory effect and eucalyptus extract with mean of %21/ 92 had the least inhibitory effect on M. fructigena growth rate. Also, by increasing the extract concentration of each herb (garlic, mint, thyme, eucalyptus and oleander), the M. fructigena growth rate was reduced. In general, due to the best inhibitory effect of garlic and oleander extract with 20 mg/l concentration, it is recommended to be used in practical experiments to control the causative agent of brown rot in stone fruit trees.