بررسی اثر ساپونین‏ خام چند اکوتیپ‏ یونجه بر فعالیت زیستی نماتد ساقه و پیاز (Ditylenchus dipsaci)

Alfalfa stem nematode is one of the important pathogenic factors of alfalfa. In this experiment, the effect of crude saponin on six alfalfa ecotypes including Hamedani, Krisari, Feyz, Polycross of Shiraz, Mahalie of Miandoab and Mahalie of Neyshabouri on the biological activity of alfalfa stem nematodes that were propagated in vitro was investigated. The experiment was performed as a factorial design in a completely randomized design. Raw saponin of each ecotype was extracted from aerial parts including leaves and stems (Golawska et al., 2012). Then, the effect of three treatments including saponins extracted of each ecotype at two levels of 50 and 90 μl and distilled water as a control (i.e. no saponin), on the biological activity of 1500 larvae and adult nematodes was evaluated in three replications during 24 hours. The results of mean comparison showed that the application of 50 and 90 μl doses of crude saponin of Hamedani ecotype had the highest effect with 87.47 and 95.93%, respectively and the local ecotype of Miandoab with 50.87 and 87.1%, respectively had the lowest effect on death of adults and larvae of this nematodes.