بررسی مراحل توسعه‌ی نماتد ریشه‌گرهی Meloidogyne javanica در ارقام خیلی حساس و نسبتا مقاوم گوجه‌فرنگی

Studying the developmental stages of root-knot nematodes in resistant and tolerant cultivars and comparing them with susceptible cultivars can help determine the cause of resistance or susceptibility of plants. Therefore, to evaluate the developmental stages of the disease in Mobil as a highly susceptible cultivar and ALYSTE F-1 as a moderately resistant cultivar, about 3000 second stage juveniles (J2s) of Meloidogyne javanica were inoculated to each plant. Then, the roots of each cultivar (with three replicates) were separated from the soil daily for staining. This operation continued until 30 days after inoculation. The experiment was performed at 17-25 °C under greenhouse conditions. The samples were stained with acid fuchsin and examined under a light microscope. The results showed that in the highly susceptible cultivar, the highest number of pre-parasitic J2 (338.33 ± 12.73), parasitic J2 (367.67 ± 21.36), J3 (310 ± 8.35), J4 (303.33 ± 6.96) and female nematodes (326.67 ± 12.91) were observed at 3, 7, 14, 20 and 28 days after inoculation of root-knot nematodes, respectively. However, in the moderately resistant cultivar, the highest number of pre-parasitic J2 (29 ± 0.58), parasitic J2 (30 ± 0.58), J3 (16 ± 2.52), J4 (14 ± 0.58) and female nematodes (1.67 ± 1.67) were recorded at 4, 9, 19, 25 and 28 days after inoculation, respectively. In the moderately resistant cultivar ALYSTE F-1, the number of invasive J2 was significantly lower than in the highly susceptible cultivar Mobil. The results also showed that the ALYSTE F-1 cultivar interfered in the development of nematode life stages and increased the life period of M. javanica. Thus, it was determined that ALYSTE F-1 plays a role in reducing nematode reproduction.