کنترل بیولوژیک بیماری کپک خاکستری توت‌فرنگی ناشی از Botrytis cinerea با استفاده از Leptosphaerulina australis جدا شده از فیلوسفر توت‌فرنگی

Botrytis cinerea is one of the most important strawberry pathogens, which caused strawberry gray mold disease. In order to study, of phyllosphere and phylloplane microbial community of Fragaria anannasa, numerous samplings were conducted from healthy strawberry plants (without disease symptoms) grown in different areas in Jiroft, during the summer and winter of 2018. After surface sterilization (dipping in 2% sodium hypochlorite for 2 min, and rinsing three times in distilled water) and drying in a sterile filter paper, samples were plated on PDA. During isolation one isolate of Leptosphaerulina obtained from strawberry healthy leaves. The biocontrol effects of this fungus were evaluated in dual-culture and volatile metabolites against Botrytis cinerea. As preliminary results, the fungus inhibited mycelial growth with inhibition zone 4 mm in dual-culture and 51/51% in the volatile. Based on morphological traits such as the characteristics of pseudothecia, asci and ascospores and molecular data of ITS-rDNA, the fungus was identified as Leptosphaerulina australis. According to our knowledge, this is the first report of the association of this fungus with the aerial parts of the strawberry plant. Necessary studies are underwaying to determine the efficiency of the biocontrol agent in greenhouse conditions