تغییر بیان ژن CYP6EC1 و ژن‌های گیرنده‌ی انتقال دهنده‌های عصبی گابا و گلوتامات در تریپس غربی گل مقاوم به آبامکتین

The control of western flower thrips as one of the main pests of agricultural products is a serious problem due to the limited number of insecticides available for it. Researches have shown that one of the main mechanisms of resistance to abamectin, as a common insecticide in the control of western flower thrips, is the increase in cytochrome P450 activity. Also, changes in Rdl-GABA and GluCl expression have been reported as the causes of resistance to this insecticide. In this study, the role of two genes from the CYP4 and CYP6 families, including CYP6EB1 and CYP6EC1, as well as Rdl-GABA and GluCl was evaluated in the resistance of western flower thrips to abamectin through real-time PCR. Based on the results, it was found that there is no difference in the expression of CYP6EB1 between resistant and susceptible populations, while the expression of CYP6EC1 in the resistant population was significantly increased by 3.006-fold. Also, the expression analysis of Rdl-GABA and GluCl showed that in the resistant population, there was a significant decrease of 0.497 and 0.406, respectively. Over-expression of CYP6EC1 showed that this family of cytochrome P450 is involved in the resistance of western flower thrips to abamectin. It can also be interpreted that down-regulation of Rdl-GABA and GluCl in resistant population has reduced the binding of abamectin to its target site and reduced the toxicity. Therefore, the obtained results confirm that an increase in CYP6EC1 expression and the a decrease in Rdl-GABA and GluCl expression, are the main mechanisms of resistance to abamectin in the resistant population of western flower thrips.