وجود آلودگی مخلوط مزارع کدو به چند ویروس مهم در مزارع جالیز کاری استان زنجان

Plants viruses are one of the most important factor causing high yield losses of cucurbit crops around the world and Iran. Yellowing and mosaic are the most symptoms observing in infected plants. In this research, to evaluate mixed infection of several important plant viruses, samples showing yellowing and mosaic symptoms were collected from the main cucurbit fields in the south west of Zanjan province during summer and fall 2019. The suspected samples were evaluated to mix infection by RT-PCR. To aim, total RNAs were extracted and corresponded to synthesis the cDNAs using specific primers. Polymerase Chain Reaction (PCR) was optimized using a parir specific primer for each viruses including Cucumber mosaic virus, Zuchini yellow mosaic virus, Cucumber green mottle mosaic virus and a universal primer to detect ortothospovirus members. The results revealed that mixed infection in five samples in different fields. The results confirmed that the sample Fg and Fm isolated in cucurbits fields are infected by Zucchini yellow mosaic virus (ZYMV) and a member of ortothospovirus. Mixed infection ZYMV and CMV was detected in sample Fw that isolated from Zucchini host. Samples Ic and Id showed mixed infection of CMV and Cucumber green mottle mosaic virus (CGMMV). ZYMV and CMV were the most prevalent viruses that detected individually and mixed with other viruses in cucurbit fields. In PCR the fragments were amplified by specific primers in 500, 650, 550 and 700 bp in length related to ZYMV, CMV, CGMMV and Orthotospovirs members respectively. Different symptoms were observed in cucurbits fields. The relation between detected viruses and symptoms should be investigate