بررسی اثر ضد ویروسی عصاره آبی و اتانولی زیره سبز در حذف ویروس لکه حلقوی بافت مرده هسته داران از گل محمدی در محیط کشت

Cuminum cyminum is a flowering plant in the family Apiaceae, native from the east Mediterranean to South Asia. Cumin's distinctive flavour is strong. It has a warm aroma due to its essential oil content. Its main constituent aroma compounds are cuminaldehyde and cuminic alcohol. This research focused on the use of C. cyminum aquatic and ethanolic extracts for the production of virus-free damask rose by explant culture from infected parent material. Toward this aim a survey was conducted to detect the viruses infecting Rosa damascena in Markazi province (Naragh). Using RT-PCR and Ilar1 و Ilar2 primers, 180 samples were tested for the presence of Prunus necrotic ringspot virus (PNRSV). Transplant medium culture in MS medium was performed for a 12 infected damask rose transplants, pieces 8 cm long and 1 cm in diameter from the budding stem which were infected with PNRSV viruses. Dried powdered seeds of C. cyminum plants were extracted with water and 95% ethanol. Aquatic and ethanolic C. cyminum extracts inoculated with three different concentrations (5, 2, 0.5 mg/ml) (extract/ml medium) into MS media. Damask rose transplants were tested using RT-PCR for the presence of virus 30 days after treating. Results indicated that the aquatic extract of C. cyminum at the concentration of 5 and 2 mg/ml and ethanolic extract at the concentration of 0.5 and 2 (mg/ml) are effective in eliminating 75% of PNRSV. Also, average results of absorption of DAS-ELISA using specific antibody test were shown alcoholic extracts of C. cyminum is more effective than aquatic extract. According to the results of this study C. cyminum aquatic and alcoholic extracts are able to adversely affect the rose mosaic virus replication. They may be used as an herbal source as viricidal preparations against PNRSV.