ردیابی ژن Hs1pro-1 ، عامل احتمالی مقاومت به نماتد سیست چغندرقند در تعدادی از ژنوتیپ‌های شناخته شده در ایران

Sugar beet (Beta vulgaris sub sp. vulgaris) is a biennial plant from the family Amaranthaceae that is cultivated in temperate and subtropical regions. This crop is economically important because of the production of about one-third of the globally consumed sugar. The beet cyst nematode (BCN), Heterodera schachtii, is one of the most important pathogens of sugar beet, common in most cultivation areas around the world, including Europe and North America.. The most effective and economical method to manage this nematode is using resistant and tolerant cultivars. There is no source of resistance to BCN in cultivated sugar beets, but resistance to BCN in some commercial beet lines like Nemakill, Evasion, Nematop has been achieved through an interspecific cross between the beet and its relative Patellifolia procumbens. According to the available data, the resistance of beet against this nematode is attributed to Hs1pro-1 gene. The present study was performed to detect Hs1pro-1 gene in some Iranian genotypes. For this, 20 resistant genotypes were obtained from Sugar Beet Research Institute, Karaj and DNA was extracted from foliar samples using CTAB method. The polymerase chain reaction (PCR) was performed using the specific primer pairs, P11-F and M12-R and the results of gel electrophoresis of PCR products showed the presence of an 820 bp fragment in the cultivars Arya, Sanetta and Bifort, and the lines S1_920833 and S1_960015. Although, the Hs1pro-1 has been cloned as the first BCN resistance gene, its role in conferring resistance against BCN has not yet been proved and need more investigations. Here, the correlation between the observed reactions of the selected studied genotypes and lines, and the presence of the aforementioned gene was discussed.