Nanoencapsulation of monoterpenoids 1,8-cineole and thymol by zeolite A4 to enhance ‎their fumigant toxicity persistence against Tetranychus urticae Koch
Paper ID : 1632-24IPPC (R2)
Authors
1دانشکده کشاورزی و منابع طبیعی مغان، دانشگاه محقق اردبیلی، اردبیل،ایران
2گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه گیلان، ایران
Abstract
Due to detrimental side-effects of synthetic pesticide application such as environmental contamination and the development of pest resistance, an interest in botanical-based pesticides has increased in recent years. The pesticidal effects of plant essential oils and their main components especially monoterpenoids have been reported on several groups of insects and mites. However, based on sensitivity to the light, oxygen and high temperatures, these agents are environmentally unstable. Therefore, introducing novel formulations to enhance their stability may be ideal. In the present study, 1,8-cineole and thymol monoterpenoids, introduced by high pesticidal potential, were nanoencapsulated by the mesoporous material zeolite A4 to enhance the stability of fumigant toxicity against two spotted spider mite (Tetranychus urticae Koch). Seventy percentage lethal concentrations (LC70) were selected for nanoencapsulation of 1,8-cineole and thymol; 6.07 µl/l and 4.28 mg/l, respectively. The encapsulation efficiency of 1,8-cineole and thymol in zeolite A4 were calculated as 93.45 ± 1.50% and 94.17 ± 0.83%. The encapsulated monoterpenoids was assessed by SEM and their nano size (less than 100 nm) was distinguished. Nanoencapsulation of 1,8-cineole and thymol by zeolite A4 prolonged the stability of both monoterpenoids so that their fumigant toxicity persistence from 4 and 6 days in pure formulations reached to 25 and 32 days for nanoencapsulated ones, respectively. According to the results of present study, the nanoencapsulation of 1,8-cineole and thymol by zeolite A4 is an appropriate technique to overcome their low fumigant stability and can be recommended for further studies in the management of T. urticae.
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