بررسی تاثیر اسانس پوست لیموترش Citrus Limon L بر سلول های خونی لارو پروانه موم خوار(Galleria mellonella

Galleria mellonella is one of the most important pests of agricultural products in Iran. Due to the adverse effects of chemical pesticides, the need for alternative methods is inevitable. Essential oils and plant extracts are suitable candidates as alternatives to synthetic chemical compounds. Blood cells play an important role in the insect immune system and protect them against environmental stress or chemical and microbial toxins, so the study and recognition of these cells in response to essential oils can be an effective step in assessing the extent and effect of essential oil on Show pest. This study was performed to investigate the effect of essential oil on the pest defense system. In this experiment, the total number of blood cells, granulocytes and plasmotocytes of (G. mellonella) were calculated after injection of lemon essential oil at intervals of 3, 6, 12 and 24 hours. The results showed that with increasing concentration, the number of plasmotocytes cells increased and the number of granulocytes and the total number of cells showed a variable trend. The presence of the pathogen in the hemolymph of insects can activate their immune system and cause changes in the total number of blood cells. The decrease in the number of blood cells can be related to the formation of nodules and capsules around the invaders as well as the effect of secondary metabolites in some pathogens. . However, the increase in the number of blood cells in some cases can be due to the stimulation of hemopoiesis due to the presence of pathogens in the blood. Changes in plasmotocytes counts are due to the loss of blood cells or may be due to cell defense activity. Blood cells treated in vitro under the influence of essential oil became nodular or cumulative. Changes in the number of granulocytes indicate their function in activating the insect defense system against foreign objects.