بررسی امکان کنترل بیولوژیکی قارچ Ascochyta rabiei توسط قارچ آنتاگونیست Trichoderma در شرایط آزمایشگاه و گلخانه

Abstract

In this study, three trichoderma isolates were prepared from the laboratory collection of the Faculty of Agriculture, Ferdowsi University, for biocontrol tests in laboratory and greenhouse conditions. The potential of Trichoderma isolates in the control of Ascochyta rabiei and lightning disease in laboratory and greenhouse was evaluated in a completely randomized design with four replications. For this purpose, selected isolates were studied in vitro using cross-culture methods, volatile compounds and non-volatile compounds in the fungus. In all the mentioned experiments, the causative fungus was measured 168 hours after inoculation. The results showed that petri dishes containing isolates kept in an incubator at 25 ° C significantly reduced the growth of the fungus. In all the mentioned experiments, the causative fungus was measured 168 hours after inoculation. The results showed that petri dishes containing isolates kept in an incubator at 25 ° C significantly reduced the growth of the fungus. In the study of non-volatile metabolites of Trichoderma atroviride caused the greatest reduction in pathogen growth by 80%.And under the influence of volatile metabolites Trichoderma viride with the least inhibitory effect of 8.43% reduced the growth of the pathogen. Trichoderma isolates were propagated using culture medium containing wheat bran and their effect in greenhouse conditions on the control of pea blight was evaluated. Trichoderma isolates were evaluated in the form of coating of chickpea seeds and in soil contaminated with pathogenic fungi in terms of control of lightning disease in four replications. The severity of the disease was assessed three weeks after inoculation. Disease severity was recorded using a 9-degree index. According to the results, Trichoderma viridae was the most effective isolate in terms of reducing the disease of chickpea blight in greenhouse conditions. The general results of this study show that it is possible to control the important disease of pea blight using Trichoderma antagonist fungus.