بررسی جذب عنصر مس توسط دو قارچ Trichoderma harzianum و Paraconiothyrium brasiliense در محیط کشت مایع

The environmental pollution is one of the most important problems of human societies in the 21st century. The most important of these pollutants are heavy metals such as copper (cu). The concentration of heavy metals in environment are increasing due to various industrial and agricultural activities in nature. Recently, absorption of heavy metals by microorganisms (biological absorption) has been introduced as the most effective method for decontamination of effluents containing heavy metals and rapid reduction of toxin concentrations in effluents. Among them, fungi have more absorption capacity than other microorganisms. The unique property of fungi is binding ability of cations and anions to their cell wall. Therefore, this study was performed to investigate the understanding copper absorption of Trichoderma harzianum and Paraconiothyrium brasiliense in liquid culture medium. First, each fungus was cultured separately in fungi liquid culture medium (PDB) containing 60 ppm cu in three replications. Then the fungi reached their maximum growth at the desired temperature for 20 days. During this time, four samples were collected with five days intervals to evaluate elemental contents using ICP equipment. The results showed that Cu absorption increased by both fungi from the first stage to the end of sampling. The maximum absorption was observed in the fourth stage and the residual element was minimized in the environment. Paraconiohygrium brasiliense with a residue of about 20 ppm of Cu in culture showed more absorption ability than Trichoderma harzianum (with a residue of about 35 ppm). In general, both studied fungal species are recommended for further studies of Cu bioremediation.