متابولیت های ثانویه برخی قارچ های جدا شده از خاک های جنگلی با استفاده از GC-MS

Fungi are one of the most important soil microorganisms. Study on soil fungi and their secondary metabolites allows us to use them in different fields. In this study, the secondary metabolites of four soil fungi including Bionectaria sp., Pochonia chlamydosporia, Microsphaeropsis arundinis and Scupulariopsis atematum that were isolated and identified from the forest soils of Amol city were studied. The fungi were cultured in PDB medium and after two weeks, the medium was filtered through filter paper and mixed with 20 cc of ethyl acetate and shaked well. After centrifugation and separation of the supernatant, the solvent was evaporated by rotory evaporator and sediment were subjected to GC-MS. After analysis of the obtained chromatograms, several compounds were identified, including hexadecanoic acid methyl ester, phenol, octadecane, eicosane and 1,2-benzenedicarboxylic acid. The most important compounds, including tetracosans in the culture medium of Bionectaria sp. and Scupulariopsis altematum were observed. Tetracusan is a straight-chain alkane, which contains 24 carbon atoms and is present as a plant metabolite and a volatile component in oils. Isopropyl myristate was found in Microsphaeropsis arundinis. Isopropyl myristate is an emollient and is used in the preparation of topical medicines and cosmetic creams and is well absorbed by the skin. This compound is used as a therapist against body lice as well as a solvent in perfumery and in mouthwash formulas to control oral bacteria. These compounds are reported for the first time from mentioned fungi.