تولید مایکوتوکسین زرالنون توسط جدایه‌های Fusarium graminearum همراه جیره غذایی مرغ

More than 90% of chicken rations are prepared from abroad and in different countries. Corn is the main component of chicken diet. Fusarium graminearum is one of the main pathogens of cereals in the field. It is a generator of a variety of mycotoxins such as Zearalenone, deoxy-nivalnol, T2, and fusaric acid. In this study, mycotoxin production was investigated by isolates of Fusarium graminearum. At first, dietary samples were prepared from several provinces of the country and fungi were isolated. Morphological identification of the species was performed using standard potato culture medium of dextrose agar and Carnation leaves agar in dark conditions and light rotation of 12-12 hours, respectively, based on valid laboratory keys. Molecular identification was performed with a specific TEF primer. Thus, Fusarium graminearum isolates were identified. In order to investigate the production of toxin, two isolates were selected and added to the standard culture medium and then to the standard toxin culture medium and kept in standard conditions (25 Celsius). The mycotoxin Zearalenone was extracted from the culture medium. . To detect mycotoxin production, the extract prepared by HPLC was tested. For two isolates of Fusarium graminearum, the levels of ppb61 and ppb78 of the mycotoxin zeralnone were detected, respectively. This highlights the importance of studying dietary fungi and the toxins they produce because of their impact on society.