فراوانی قارچ Fusarium proliferatum در جیره غذایی مرغ چند استان کشور

Given that today over 90% of chicken diets are produced from abroad and in different countries, determining the fungal flora along the diet is a must. Maize is the main component of chicken diet. Fusarium proliferatum is one of the major corn field pathologists. In this study, five regions of Lorestan, Isfahan, Zanjan and Alborz provinces were sampled. After transferring the samples to the laboratory, isolates of F. proliferatum isolates were isolated by means of isolation method using two methods of suspension and plant growth on pepon pentachloronitrobenzene agar (PPA). Morphological identification of the species was carried out using standard culture Potato Dextrose Agar (PDA) and Carnation Leaf Agar (CLA) culture medium in terms of darkness and optoelectronic 12-12 hours, respectively, based on valid chi-square tests. Molecular identification was performed with specific primer Proli1F and TEF1R. The results indicate that Alborz province with 40 isolates and 50% abundance and then Lorestan provinces with 18 and 23% frequency, Isfahan 13 and 16% frequency, and Zanjan with 9and frequency 11%, respectively. The corn of the three provinces of Lorestan, Zanjan and Esfahan was generally imported from Brazil and Argentina, while corn from the province of Alborz was imported from Poland. This issue indicates the importance and role of the fungus F. proliferatum ecology, as well as the quality and use of methods for the elimination of corn contamination imported into the country. F. proliferatum have genetic clusters that produce the enzyme profile necessary for the production of mycotoxins such as fumonisin and moniliformin, which produces these mycotoxins in appropriate conditions. These mycotoxins will have various negative effects on animals and humans through the use of mycotoxin-contaminated products.