جدا سازی و شناسایی قارچ های خاکزی جنگلی از حومه آمل

Fungi are one group of microorganisms that can be found in the soil of different regions. Soil fungi are important in several ways. These microorganisms in the soil can cause pathogenesis and there are also some mycorrhizal fungi that can be beneficial to plants. In this study, soil samples from a depth of 0-15 cm of surface soil were gathered from several forest regions around Amol city. First, a soil suspension was prepared in a dilution of one to 5000 and cultured in a medium containing Rose-Bengal and streptomycin sulphate. After growth of colonies in rose Bengal medium, they were purified by hyphal tip and single spore methods and transferred to fresh PDA slants. Following preliminary morphological studies, the harvested isolates were subjected to molecular identification based on sequence data of ITSrDNA genomic region. After preparing the resulted sequences and NCBI BLAST search, seven species including Aspergillus heterothallicus, Aspergillus tritici, Chaetomium globosum, Clonostachys rossmaniae, Hypoxylon investiens, Phaeoacremonium fraxinopennsylvanicum and Staphylotrichum coccosporum were identified. Among them, three species including A. heterothallicus, A. tritici, Cl. rossmaniae and, genus and species S. coccosporum, reported as new records for the funga of Iran.