مطالعه تاثیر ساپونین های‏ خالص چند اکوتیپ‏ یونجه (Medicago sativa L.) بر مرگ و میر نماتد ساقه یونجه

Alfalfa stem nematode (D. dipsaci) is one of the factors reducing the yield of alfalfa and some crops, ornamental and rangeland plants. In this study, the effects of pure saponin on six alfalfa ecotypes including Polycross of Shiraz, Hamedani, Feyz, Krisari, local of Miandoab and Neyshabouri on alfalfa stem nematode activity was evaluated. The test was performed in a completely randomized design in factorial test, on larval and adult nematodes. Pure saponin was extracted from leaves and stems of each alfalfa ecotypes (Golawska et al., 2012). The effect of 10 and 50 μl levels of pure saponin and distilled water (as a control treatment) on the mortality of 1500 larvae and adult nematodes was investigated in three replications, after 24 hours. The results of statistical calculations showed that the pure saponin of Hamedani ecotype had the highest effective with 96 and 99.1% in doses of 10 and 50 μl, respectively and the pure saponin of local ecotype of Miandoab of with 79.78 and 96.1 in doses of 10 and 50 μl, respectively had the lowest effect on the mortality of larval and adult stages of D. dipsaci.