سمیت سلولی دپسی پپتید‌های حلقوی و پپتید‌های خطی باکتری جنس Xenorhabdus، روی رده‌های سلولی تومور موش (B16)، سرطان پستان (MCF-7) و سرطان ریه (A549).

Cancer is one of the most common diseases in the world, that starts at one point in the body and if diagnosed late, it can affect the whole body. The effectiveness of drug treatments is low, but their side effects are very high. This experimental study aims to evaluate the in vitro cytotoxic effects of ethyl acetate extracts from four entomopathogenic bacteria (EPB) including Xenorhabdus nematophila FUM 220 (1), Xenorhabdus nematophila FUM 221 (2), Xenorhabdus bovienii FUM 223 (3), and Xenorhabdus bovienii FUM 222 (4). Three compounds, including xenortide-410 (5), xenematide A 663 (6), and nematophin-273 (7) were isolated from X. nematophila FUM 220. In this study, samples 1 to 7 were used to treat murine tumor (B16), human breast cancer (MCF-7), and human lung cancer (A549) cell lines. The cytotoxicity of samples were assessed using an AlamarBlue®. The results demonstrated that among the bacterial extracts, potent cytotoxicity was found for samples 1 and 3 against A549 (IC50 = 4.3 and 2.4 µg/mL), respectively. Samples 1 and 3 were characterized by strong cytotoxic activities against MCF-7 cells with IC50 values of 6.6 and 6.7 μg/mL, respectively, comparable to that of doxorubicin (IC50 = 0.07 μg/mL). In addition, it should be noted that samples 1, 3, and 4, are also significantly active against B16 cells, with IC50 values of 3.79, 2.62, and 2.64 μg/mL, respectively. Strong cytotoxic activity was found for samples 6 and 7 with IC50 of 6.2 μg/mL against A549 cells. The findings of this study indicate that, Xenorhabdus sp. produce compounds with potent cytotoxic activity. Useful compounds could be isolated, identified, commercialized and used to develop new chemotherapy agents to treat cancer.